In silico and empirical evaluation of twelve metabarcoding primer sets for insectivorous diet analyses

Orianne Tournayre, Ondine Filippi-Codaccioni, Marine Trillat, Sylvain Piry, Dominique Pontier, Nathalie Charbonnel, Maxime Galan & Maxime Leuchtmann
During the most recent decade, environmental DNA metabarcoding approaches have been both developed and improved to minimize the biological and technical biases in these protocols. However, challenges remain, notably those relating to primer design. In the current study, we comprehensively assessed the performance of ten COI and two 16S primer pairs for eDNA metabarcoding, including novel and previously published primers. We used a combined approach of in silico, in vivo-mock community (33 arthropod taxa from...
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7 downloads reported since publication in 2019.

These counts follow the COUNTER Code of Practice, meaning that Internet robots and repeats within a certain time frame are excluded.
What does this mean?