Data from: Tracking transcription factor mobility and interaction in Arabidopsis roots with fluorescence correlation spectroscopy

Natalie M Clark, Elizabeth Hinde, Cara M Winter, Adam P Fisher, Giuseppe Crosti, Ikram Blilou, Enrico Gratton, Philip N Benfey & Rosangela Sozzani
To understand complex regulatory processes in multicellular organisms, it is critical to be able to quantitatively analyze protein movement and protein-protein interactions in time and space. During Arabidopsis development, the intercellular movement of SHORTROOT (SHR) and subsequent interaction with its downstream target SCARECROW (SCR) control root patterning and cell fate specification. However, quantitative information about the spatio-temporal dynamics of SHR movement and SHR-SCR interaction is currently unavailable. Here, we quantify parameters including SHR mobility, oligomeric...
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