Data from: An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii

Xiaobo Li, Ru Zhang, Weronika Patena, Spencer S. Gang, Sean R. Blum, Nina Ivanova, Rebecca Yue, Jacob M. Robertson, Paul A. Lefebvre, Sorel T. Fitz-Gibbon, Arthur R. Grossman & Martin C. Jonikas
The green alga Chlamydomonas reinhardtii is a leading single-celled model for dissecting biological processes in photosynthetic eukaryotes. However, its usefulness has been limited by difficulties in obtaining mutants in genes of interest. To allow generation of large numbers of mapped mutants, we developed high-throughput methods which: (1) Enable easy propagation on agar and cryogenic maintenance of tens of thousands of C. reinhardtii strains; (2) Identify mutant insertion sites and physical coordinates in such collections; (3)...
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