Long- and short-read metabarcoding technologies reveal similar spatio-temporal structures in fungal communities

Brendan Furneaux, Mohammad Bahram, Anna Rosling, Nourou Yorou & Martin Ryberg
Fungi form diverse communities and play essential roles in many terrestrial ecosystems, yet there are methodological challenges in taxonomic and phylogenetic placement of fungi from environmental sequences. To address such challenges we investigated spatio-temporal structure of a fungal community using soil metabarcoding with four different sequencing strategies: short amplicon sequencing of the ITS2 region (300--400\ bp) with Illumina MiSeq, Ion Torrent Ion S5, and PacBio RS II, all from the same PCR library, as well...
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