Assembly of Aegilops umbellulata chromosomes

Michael Abrouk, István Molnár, Mihaela Martis, Eva Hřibová, Hana Šimková, Jan Vrána, András Farkas, Szabolc Makai, Angéla Juhász, Burkhard Steuernagel, Marie Kubaláková, Klaus Mayer, Márta Molnár-Láng, Jaroslav Doležel & Jan Bartoš
We sequenced and assembled seven chromosomes of Aegilops umbellulata. The assembly with Meraculous resulted in ~ 101k - 191k scaffolds per chromosome with N50 size 2.9kb – 4.0kb. The scaffold sequences were used for development of molecular markers specific for cDNAs sequences mapped on Ae. umbellulata chromosomes Pairwise alignment of wheat cDNA-sequences and the chromosomal scaffolds of Ae. umbellulata identified candidate sequences. In order to analyze the structure and homeology of Aegilops chromosomes, forty-three mapped...
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