Data from: Serum-free and xenobiotic-free preservation of cultured human limbal epithelial cells

Oeygunn A. Utheim, Rakibul Islam, Torstein Lyberg, Borghild Roald, Jon Roger Eidet, Maria Fideliz De La Paz, Darlene A. Dartt, Sten Raeder, Tor Paaske Utheim & Oeygunn Utheim
Aim/Purpose of the Study: To develop a one-week storage method, without serum and xenobiotics, that would maintain cell viability, morphology, and phenotype of cultured human limbal epithelial sheets. Materials and Methods: Human limbal explants were cultured on intact human amniotic membranes for two weeks. The sheets were stored in a hermetically sealed container at 23°C in either a serum-free medium with selected animal serum-derived compounds (Quantum 286) or a xenobiotic-free medium (Minimal Essential Medium) for...
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