How to quantify factors degrading DNA in the environment and predict degradation for effective sampling design

Thomas Naef, Anne-Laure Besnard, Lisa Lehnen, Eric J. Petit, Jaap Van Schaik & Sebastien J. Puechmaille
Extra-organismal DNA (eoDNA) from material left behind by organisms (non-invasive DNA: e.g., faeces, hair) or from environmental samples (eDNA: e.g., water, soil) is a valuable source of genetic information. However, the relatively low quality and quantity of eoDNA, which can be further degraded by environmental factors, results in reduced amplification and sequencing success. This is often compensated for through cost- and time-intensive replications of genotyping/sequencing procedures. Therefore, system- and site-specific quantifications of environmental degradation are...
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