Data from: Demodifying RNA for transcriptomic analyses of archival formalin-fixed paraffin-embedded samples

Leah C. Wehmas, Charles E. Wood, Remi Gagne, Andrew Williams, Carole Yauk, Mark M. Gosink, Deidre Dalmas, Ruixin Hao, Raegan O'Lone & Susan Hester
Archival formalin-fixed paraffin-embedded (FFPE) tissue samples offer a vast but largely untapped resource for genomic research. The primary technical issues limiting use of FFPE samples are RNA yield and quality. In this study, we evaluated methods to demodify RNA highly fragmented and crosslinked by formalin fixation. Primary endpoints were RNA recovery, RNA-sequencing quality metrics, and transcriptional responses to a reference chemical (phenobarbital, PB). Frozen mouse liver samples from control and PB groups (n=6/group) were divided...
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