Primer biases in the molecular assessment of diet in multiple insectivorous mammals

Samuel Browett, Thomas Curran, Denise O'Meara, Andrew Harrington, Naiara Guimarães Sales, Rachael Antwis, David O'Neill & Allan McDevitt
Our understanding of trophic interactions of small insectivorous mammals has been drastically improved with the advent of DNA metabarcoding. The technique has continued to be optimised over the years, with primer choice repeatedly being a vital factor for dietary inferences. However, the majority of dietary studies examining the effect of primer choice often rely on in silico analyses or comparing between species that occupy an identical niche type. Here we apply DNA metabarcoding to empirically...
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