Data from: Y-box protein 1 is required to sort microRNAs into exosomes in cells and in a cell-free reaction

Matthew J. Shurtleff, Morayma M. Temoche-Diaz, Kate V. Karfilis, Sayaka Ri & Randy Schekman
Exosomes are small vesicles that are secreted from metazoan cells and may convey selected membrane proteins and small RNAs to target cells for the control of cell migration, development and metastasis. To study the mechanisms of RNA packaging into exosomes, we devised a purification scheme based on the membrane marker CD63 to isolate a single exosome species secreted from HEK293T cells. Using immunoisolated CD63-containing exosomes we identified a set of miRNAs that are highly enriched...
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