Data from: Cross-species transferability of SSR loci developed from transcriptome sequencing in lodgepole pine

Mark R. Lesser, Thomas L. Parchman & C. Alex Buerkle
With the advent of next generation sequencing technologies, transcriptome level sequence collections are arising as prominent resources for the discovery of gene-based molecular markers. In a previous study more than 15 000 simple sequence repeats (SSRs) in expressed sequence tag (EST) sequences resulting from 454 pyrosequencing of Pinus contorta cDNA were identified. From these we developed PCR primers for approximately 4000 candidate SSRs. Here, we tested 184 of these SSRs for successful amplification across P....
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