Gene Delivery to Neurons in the Auditory Brainstem of Barn Owls using Standard Recombinant Adeno-associated Virus Vectors

Nadine Thiele, K. Jannis Hildebrandt & Christine Köppl
Recombinant adeno-associated virus (rAAV) vectors are a commonly used tool for gene delivery. There is a large choice of different serotypes whose transduction efficiency varies for different animal species. In this study, three rAAV vectors were tested for transduction efficiency in the auditory brainstem of adult barn owls (Tyto alba) which are not standard laboratory animals. Injections with rAAV serotypes 2/1 and 2/5 resulted in reliable expression in various nuclei of the auditory brainstem of...
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