Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation

Lindsay Case & Michael Rosen
Integrin adhesion complexes (IACs) are integrin-based plasma membrane-associated compartments where cells sense environmental cues. The physical mechanisms and molecular interactions that mediate initial IAC formation are unclear. We found that both p130Cas (“Cas”) and Focal adhesion kinase (“FAK”) undergo liquid-liquid phase separation in vitrounder physiologic conditions. Cas- and FAK- driven phase separation is sufficient to reconstitute kindlin-dependent integrin clustering in vitro with recombinant mammalian proteins. In vitro condensates and IACs in mouse embryonic fibroblasts (MEFs)...
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