Data from: Novel insights into relationships between egg corticosterone and timing of breeding revealed by LC-MS/MS

Tom Rosendahl Larsen, Graham D. Fairhurst, Siegrid De Baere, Siska Croubels, Wendt Müller, Liesbeth De Neve & Luc Lens
Inter- and intra-clutch variation in egg corticosterone (CORT), the major glucocorticoid in birds, may provide insights into how maternal stress levels vary with the timing of breeding and with laying order. Common analytical methods (e.g. immunoassays), however, suffer from cross-reaction with other steroids, leading to potential overestimation of CORT concentrations which can obscure true hormone–environment relationships and complicate among-study comparisons. We here apply a new LC-MS/MS technique, which has recently been shown to avoid the...
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