Intravital quantification reveals dynamic calcium concentration changes across B cell differentiation stages

Carolin Ulbricht, Ruth Leben, Asylkhan Rakhymzhan, Frank Kirchhoff, Lars Nitschke, Helena Radbruch, Raluca Niesner & Anja Hauser
Calcium is a universal second messenger present in all eukaryotic cells. The mobilization and storage of Ca2+ ions drives a number of signaling-related processes, stress-responses or metabolic changes, all of which are relevant for the development of immune cells and their adaption to pathogens. Here, we introduce the FRET-reporter mouse YellowCaB expressing the genetically encoded calcium indicator TN-XXL in B lymphocytes. Calcium-induced conformation change of TN-XXL results in FRET-donor quenching measurable by two-photon fluorescence lifetime...
1 citation reported since publication in 2020.
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