Data from: Soil sampling and isolation of extracellular DNA from large amount of starting material suitable for metabarcoding studies

Pierre Taberlet, Sophie M. Prud'homme, Etienne Campione, Julien Roy, Christian Miquel, Wasim Shehzad, Ludovic Gielly, Delphine Rioux, Philippe Choler, Jean-Christophe Clément, Christelle Melodelima, François Pompanon & Eric Coissac
DNA metabarcoding corresponds to the DNA-based identification of multiple species from a single complex and degraded environmental sample. We developed new sampling and extraction protocols suitable for DNA metabarcoding analyses, targeting soil extracellular DNA. The proposed sampling protocol has been designed to reduce as much as possible the influence of the local heterogeneity by processing large amount of soil, resulting from the mixing of many different cores. The DNA extraction is based on the use...
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