Data from: Environmental metabarcodes for insects: in silico PCR reveals potential for taxonomic bias

Laurence J. Clarke, Julien Soubrier, Laura S. Weyrich & Alan Cooper
Studies of insect assemblages are suited to the simultaneous DNA-based identification of multiple taxa known as metabarcoding. To obtain accurate estimates of diversity, metabarcoding markers ideally possess appropriate taxonomic coverage to avoid PCR-amplification bias, as well as sufficient sequence divergence to resolve species. We used in silico PCR to compare the taxonomic coverage and resolution of newly designed insect metabarcodes (targeting 16S) with that of existing markers (16S and COI) and then compared their efficiency...
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