Environmental DNA metabarcoding of cow dung reveals taxonomic and functional diversity of invertebrate assemblages

Eva Egelyng Sigsgaard, Kent Olsen, Morten D. D. Hansen, Oskar Liset Pryds Hansen, Toke Thomas Høye, Jens-Christian Svenning & Philip Francis Thomsen
Insects and other terrestrial invertebrates are declining in species richness and abundance. This includes the invertebrates associated with herbivore dung, which have been negatively affected by grazing abandonment and the progressive loss of large herbivores since the Late Pleistocene. Importantly, traditional monitoring of these invertebrates is time-consuming and requires considerable taxonomic expertise, which is becoming increasingly scarce. In this study, we investigated the potential of environmental DNA (eDNA) metabarcoding of cow dung samples for biomonitoring...
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