Circular dichroism spectroscopic and small-angle neutron scattering analysis of alpha-synuclein and bacteriorhodopsin in bicontinuous microemulsions

Douglas Hayes, Divina Anunciado, Ran Ye, Rachel Williams, Hugh O'Neill, Sai Venkatesh Pingali & Volker Urban
The membrane proteins (MPs) alpha-synuclein (ASYN) and bacteriorhodopsin (BR) were readily incorporated into bicontinuous microemulsions (BMEs) formed by two microemulsion systems: water/heptane/Aerosol-OT (AOT)/CK-2,13 and water/dodecane/sodium dodecyl sulfate (SDS)/1-pentanol. (CK-2,13 is an alkyl ethoxylate possessing two alkyl tail groups of carbon chain length 2 and 13 and an average degree of ethoxylation of 5.6.) MPs were encapsulated in BMEs through preparation of Winsor-III systems at optimal salinity, with the anionic surfactants AOT and SDS providing the...
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