Data from: \"A reduced representation libraries approach for nuclear marker development via 454 sequencing applied on Tetramorium (Hymenoptera: Formicidae)\" in Genomic Resources Notes Accepted 1 February 2015 to 31 March 2015

Herbert C. Wagner, Francesco Cicconardi, Gregor A. Wachter, Heike Ritthammer, Florian M. Steiner, Birgit C. Schlick-Steiner & Wolfgang Arthofer
To clarify species boundaries in the cryptic Tetramorium caespitum/impurum ant species complex, a multi-disciplinary approach is necessary. Nuclear DNA markers constitute one important step in species delimitation. We established a reduced representation library (RRL) based on amplified fragment-length polymorphism (AFLP) PCR products of four species. Amplicons were size-selected by gel-electrophoresis, DNA-fragments recovered from the agarose gels, and 454 libraries constructed by a commercial provider. Pyrosequencing yielded 490,155 reads, from which we designed cross-species amplifying primer...
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