Data from: A method to generate multi-locus barcodes of pinned insect specimens using MiSeq

Trace Akankunda, Hien To, Carlos R Lopez, Remko Leijs & Katja Hogendoorn
For molecular insect identification, amplicon sequencing methods are recommended because they offer a cost effective approach for targeting small sets of informative genes from multiple samples. In this context, high-throughput multilocus amplicon sequencing has been achieved using the MiSeq Illumina sequencing platform. However, this approach generates short gene fragments of less than 500 bp, which then have to be overlapped using bioinformatics to achieve longer sequence lengths. This increases the risk of generating chimeric sequences...
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