Data from: Effect of marker choice and thermal cycling protocol on zooplankton DNA metabarcoding studies

Laurence J. Clarke, Jason M. Beard, Kerrie M. Swadling & Bruce E. Deagle
DNA metabarcoding is a promising approach for rapidly surveying biodiversity and is likely to become an important tool for measuring ecosystem responses to environmental change. Metabarcoding markers need sufficient taxonomic coverage to detect groups of interest, sufficient sequence divergence to resolve species, and will ideally indicate relative abundance of taxa present. We characterized zooplankton assemblages with three different metabarcoding markers (nuclear 18S rDNA, mitochondrial COI, and mitochondrial 16S rDNA) to compare their performance in terms...
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