Design of a custom-made device for real-time optical measurement of differential mineral concentrations in 3D scaffolds for bone tissue engineering

Joseph Lovecchio, Valentina Betti, Marilisa Cortesi, Enrico Ravagli, Stefano Severi & Emanuele Giordano
Monitoring bone tissue engineered constructs during their maturation is important to ensure the quality of applied protocols. Several destructive, mainly histochemical, methods are conventionally used to this aim, requiring the sacrifice of the investigated samples. This implies i) to plan several scaffold replicates, ii) expensive and time consuming procedures and iii) to infer the maturity level of a given tissue construct from a cognate replica. To solve these issues, non-destructive techniques such as light spectroscopy-based...
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