The influence of intraspecific sequence variation during DNA metabarcoding: A case study of eleven fungal species

Eva Lena Estensmo, Sundy Maurice, Morgado Luis, Martin-Sanchez Pedro, Skrede Inger & Kauserud Håvard
DNA metabarcoding has become a powerful approach for analyzing complex communities from environmental samples, but there are still methodological challenges limiting its full potential. While conserved DNA markers, like 16S and 18S, often are not able to discriminate among closely related species, other more variable markers – like the fungal ITS region, may include considerable intraspecific variation, which can lead to over-splitting of species during DNA metabarcoding analyses. Here we assess the effects of intraspecific...
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These counts follow the COUNTER Code of Practice, meaning that Internet robots and repeats within a certain time frame are excluded.
What does this mean?