Standardized nuclear markers improve and homogenize species delimitation in Metazoa

Lars Dietz, Jonas Eberle, Christoph Mayer, Sandra Kukowka, Claudia Bohacz, Hannes Baur, Marianne Espeland, Bernhard A. Huber, Carl Hutter, Ximo Mengual, Ralph S. Peters, Miguel Vences, Thomas Wesener, Keith Willmott, Bernhard Misof, Oliver Niehuis & Dirk Ahrens
Species are the fundamental units of life and their recognition is essential for science and society. DNA barcoding, the use of a single and often mitochondrial gene, has been increasingly employed as a universal approach for the identification of animal species. However, this approach faces several challenges. Here, we demonstrate with empiricalgenomic data from nine metazoan animal lineages including arthropods and 28 vertebrates that multiple nuclear-encoded markers, so called universal single-copy orthologs (USCOs) performs much...
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