Data from: Massively parallel multiplex DNA sequencing for specimen identification using an Illumina MiSeq platform

Shadi Shokralla, Teresita M. Porter, Joel F. Gibson, Rafal Dobosz, Daniel H. Janzen, Winnie Hallwachs, G. Brian Golding & Mehrdad Hajibabaei
Genetic information is a valuable component of biosystematics, especially specimen identification through the use of species-specific DNA barcodes. Although many genomics applications have shifted to High-Throughput Sequencing (HTS) or Next-Generation Sequencing (NGS) technologies, sample identification (e.g., via DNA barcoding) is still most often done with Sanger sequencing. Here, we present a scalable double dual-indexing approach using an Illumina Miseq platform to sequence DNA barcode markers. We achieved 97.3% success by using half of an Illumina...
1 citation reported since publication in 2016.
601 views reported since publication in 2016.

These counts follow the COUNTER Code of Practice, meaning that Internet robots and repeats within a certain time frame are excluded.
What does this mean?
372 downloads reported since publication in 2016.

These counts follow the COUNTER Code of Practice, meaning that Internet robots and repeats within a certain time frame are excluded.
What does this mean?