Towards establishing extracellular vesicle-associated RNAs as biomarkers for HER2+ breast cancer
Colin L. Hisey, Petr Tomek, Yohanes N. S. Nursalim, Lawrence W. Chamley & Eupehmia Leung
Extracellular vesicles (EVs) are emerging as key players in breast cancer progression and hold immense promise as cancer biomarkers. However, difficulties in obtaining sufficient quantities of EVs for the identification of potential biomarkers hampers progress in this area. To circumvent this obstacle, we cultured BT-474 breast cancer cells in a two-chambered bioreactor with CDM-HD serum replacement to significantly improve the yield of cancer cell-associated EVs and eliminate bovine EV contamination. Cancer-relevant mRNAs BIRC5 (Survivin) and...
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