Data from: Nanoaperture fabrication via colloidal lithography for single molecule fluorescence analysis

Shawn H. Pfeil, Kevin Y. Chen, Yale E. Goldman, Shane A. Fiorenza, Ryan M. Jamiolkowski & Alyssa M. Tate
In single molecule fluorescence studies, background emission from labeled substrates often restricts their concentrations to non-physiological nanomolar values. One approach to address this challenge is the use of zero-mode waveguides (ZMWs), nanoscale holes in a thin metal film that physically and optically confine the observation volume allowing much higher concentrations of fluorescent substrates. Standard fabrication of ZMWs utilizes slow and costly E-beam nano-lithography. Herein, ZMWs are made using a self-assembled mask of polystyrene microspheres, enabling...
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