Micro-stepping Extended Focus reduces photobleaching and preserves structured illumination super-resolution features

Xian Hu, Salma Jalal, Michael Sheetz, Oddmund Bakke & Felix Margadant
Despite progress made in confocal microscopy, even fast systems still have insufficient temporal resolution for detailed live cell volume imaging, such as tracking rapid movement of membrane vesicles in three-dimensional space. Depending on the shortfall, this may result in undersampling and/or motion artifacts that ultimately limit the quality of the imaging data. By sacrificing the detailed information in the Z-direction, we propose a new imaging modality that involves capturing fast “projections” from the field of...
26 views reported since publication in 2020.

These counts follow the COUNTER Code of Practice, meaning that Internet robots and repeats within a certain time frame are excluded.
What does this mean?