Comparison of an extracellular vs. total DNA extraction approach for environmental DNA-based monitoring of sediment biota

Anthony Chariton, Johan Pansu, Grant Hose & Michelle Chapman
Monitoring sediment biota is an essential step for the quality assessment of aquatic ecosystems. Environmental DNA-based approaches for biomonitoring are increasing in popularity; yet, commercial kits and protocols for extracting total DNA from sediments remain expensive and time-consuming. Furthermore, they can accommodate only small amounts of sediments, potentially preventing an adequate representation of local biodiversity, especially for macro-organisms. Here, we assessed the reliability of a cost- and time- effective extracellular DNA extraction approach, able to...
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1 download reported since publication in 2021.

These counts follow the COUNTER Code of Practice, meaning that Internet robots and repeats within a certain time frame are excluded.
What does this mean?