Efficient targeted integration directed by short homology in zebrafish and mammalian cells

Jeffrey Essner & Maura McGrail
Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Here, we describe a set of resources to streamline precision gene targeting in zebrafish and demonstrate the broader utility of the method in mammalian cells. Our approach uses short homology of 24-48 bp to drive targeted integration of DNA reporter cassettes by homology-mediated end joining (HMEJ) at high frequency. Our vector series, pGTag (plasmids for Gene Tagging), contains reporters...
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