Data from: Metabarcoding using multiplexed markers increases species detection in complex zooplankton communities

Guang K. Zhang, Frédéric J.J. Chain, Cathryn L. Abbott & Melania E. Cristescu
Metabarcoding combines DNA barcoding with high-throughput sequencing, often using one genetic marker to understand complex and taxonomically diverse samples. However, species-level identification depends heavily on the choice of marker and the selected primer pair, often with a trade-off between successful species amplification and taxonomic resolution. We present a versatile metabarcoding protocol for biomonitoring that involves the use of two barcode markers (COI and 18S) and four primer pairs in a single high-throughput sequencing run, via...
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