Data from: Impacts of degraded DNA on restriction enzyme associated DNA sequencing (RADSeq)

Carly F. Graham, Travis C. Glenn, Andrew G. McArthur, Douglas R. Boreham, Troy Kieran, Stacey Lance, Richard G. Manzon, Jessica A. Martino, Todd Pierson, Sean M. Rogers, Joanna Y. Wilson & Christopher M. Somers
Degraded DNA from suboptimal field sampling is common in molecular ecology. However, its impact on techniques that use restriction site associated next-generation DNA sequencing (RADSeq, GBS) is unknown. We experimentally examined the effects of in situDNA degradation on data generation for a modified double-digest RADSeq approach (3RAD). We generated libraries using genomic DNA serially extracted from the muscle tissue of 8 individual lake whitefish (Coregonus clupeaformis) following 0-, 12-, 48- and 96-h incubation at room...
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