Data from: Indexed PCR primers induce template-specific bias in large-scale DNA sequencing studies

James L. O'Donnell, Ryan P. Kelly, Jesse A. Port, Natalie C. Lowell & James L. O’Donnell
Massively parallel sequencing is rapidly emerging as an efficient way to quantify biodiversity at all levels, from genetic variation and expression to ecological community assemblage. However, the number of reads produced per sequencing run far exceeds the number required per sample for many applications, compelling researchers to sequence multiple samples per run in order to maximize efficiency. For studies that include a PCR step, this can be accomplished using primers that include an index sequence...
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