Data from: Factors influencing detection of eDNA from a stream-dwelling amphibian

David S. Pilliod, Caren S. Goldberg, Robert S. Arkle & Lisette P. Waits
Environmental DNA (eDNA) methods for detecting and estimating abundance of aquatic species are emerging rapidly, but little is known about how processes such as secretion rate, environmental degradation, and time since colonization or extirpation from a given site affect eDNA measurements. Using stream-dwelling salamanders and quantitative PCR (qPCR) analysis, we conducted three experiments to assess eDNA: (1) production rate, (2) persistence time under different temperature and light conditions, and (3) detectability and concentration through time...
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