Biodiversity soup II

Douglas Yu, Chunyan Yang, Kristine Bohmann, Xiaoyang Wang, Cai Wang, Nathan Wales, Zhaoli Ding & Shyam Gopalakrishnan
1. Despite widespread recognition of its great promise to aid decision-making in environmental management, the applied use of metabarcoding requires improvements to reduce the multiple errors that arise during PCR amplification, sequencing, and library generation. We present a co-designed wet-lab and bioinformatic workflow for metabarcoding bulk samples that removes both false-positive (tag jumps, chimeras, erroneous sequences) and false-negative (‘dropout’) errors. However, we find that it is not possible to recover relative-abundance information from amplicon data,...
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