Modifying plant photosynthesis and growth via simultaneous chloroplast transformation of Rubisco large and small subunits

Elena Martin-Avila, Yi-Leen Lim, Rosemary Birch, Lynnette Dirk, Sally Buck, Timothy Rhodes, Robert Sharwood, Maxim Kapralov & Spencer Whitney
Engineering improved Rubisco poses a crucial strategy for enhancing photosynthesis but is challenged by the alternate locations of the plastome rbcL gene and nuclear RbcS genes. Here we develop a RNAi-RbcS Nicotiana tabacum (tobacco) master-line, tobRrΔS, amenable to rbcL-rbcS co-engineering by chloroplast transformation. Four tobacco genotypes coding alternative rbcS genes and adjoining 5ˈ-intergenic sequences revealed Rubisco production was highest in the lines incorporating a rbcS gene whose codon use and 5ˈUTR matched rbcL. These lines...
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