Data from: Combined use of molecular markers and high resolution melting (HRM) to assess chromosome dosage in potato hybrids

Clizia Villano, Valeria Miraglia, Massimo Iorizzo, Riccardo Aversano & Domenico Carputo
In plants, the most widely used cytological techniques to assess parental genome contributions are based on in situ hybridization (FISH and GISH), but they are time-consuming and need specific expertise and equipment. Recent advances in genomics and molecular biology have made PCR-based markers a straightforward, affordable technique for chromosome typing. Here, we describe the development of a molecular assay that uses single-copy conserved ortholog set II (COSII)-based single nucleotide polymorphisms (SNPs) and the high-resolution melting...
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