Data from: Evaluation of plant contamination in metabarcoding diet analysis of a herbivore

Haruko Ando, Chieko Fujii, Masataka Kawanabe, Yoshimi Ao, Tomomi Inoue & Akio Takenaka
Fecal DNA metabarcoding is currently used in various fields of ecology to determine animal diets. Contamination of non-food DNA from complex field environments is a considerable challenge to the reliability of this method but has rarely been quantified. We evaluated plant DNA contamination by sequencing the chloroplast trnL P6 loop region from food-controlled geese feces. The average percentage of contaminant sequences per sample was 1.86%. According to the results of generalized linear models, the probability...
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These counts follow the COUNTER Code of Practice, meaning that Internet robots and repeats within a certain time frame are excluded.
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