Autoantibody discovery across monogenic, acquired, and COVID19-associated autoimmunity with scalable PhIP-Seq

Sara Vazquez, Sabrina Mann, Aaron Bodansky, Andrew Kung, Zoe Quandt, Elise Ferré, Nils Landegren, Daniel Eriksson, Paul Bastard, Shen-Ying Zhang, Jamin Liu, Anthea Mitchell, Caleigh Mandel-Brehm, Brenda Miao, Gavin Sowa, Kelsey Zorn, Alice Chan, Chisato Shimizu, Adriana Tremoulet, Kara Lynch, Michael Wilson, Olle Kämpe, Kerry Dobbs, Ottavia Delmonte, Luigi Notarangelo … & Joseph DeRisi
Phage Immunoprecipitation-Sequencing (PhIP-Seq) allows for unbiased, proteome-wide autoantibody discovery across a variety of disease settings, with identification of disease-specific autoantigens providing new insight into previously poorly understood forms of immune dysregulation. Despite several successful implementations of PhIP-Seq for autoantigen discovery, including our previous work (Vazquez et al. 2020), current protocols are inherently difficult to scale to accommodate large cohorts of cases and importantly, healthy controls. Here, we develop and validate a high throughput extension of...
1 citation reported since publication in 2022.
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