Simultaneous genotyping of snails and infecting trematode parasites using high-throughput amplicon sequencing.

Cyril Hammoud, Stephen Mulero, Bert Van Bocxlaer, Jérôme Boissier, Dirk Verschuren, Christian Albrecht & Tine Huyse
Several methodological issues currently hamper the study of entire trematode communities within populations of their intermediate snail hosts. Here we develop a new workflow using high-throughput amplicon sequencing to simultaneously genotype snail hosts and their infecting trematode parasites. We designed primers to amplify 4 snail and 5 trematode markers in a single multiplex PCR. While also applicable to other genera, we focused on medically and economically important snail genera within the Superorder Hygrophila and targeted...
1 view reported since publication in 2021.

These counts follow the COUNTER Code of Practice, meaning that Internet robots and repeats within a certain time frame are excluded.
What does this mean?