Modulation of a protein-folding landscape revealed by AFM-based force spectroscopy notwithstanding instrumental limitations

Devin Edwards, Marc-Andre Leblanc & Thomas Perkins
Single-molecule force spectroscopy is a powerful tool for studying protein folding. Over the last decade, a key question has emerged: how are changes in intrinsic biomolecular dynamics altered by attachment to micron-scale force probes via flexible linkers? Here, we studied the folding/unfolding of α3D using AFM-based force spectroscopy. α3D offers an unusual opportunity as a prior smFRET study showed α3D’s molecular diffusion constant within the context of Kramers theory varies with pH. The resulting pH-dependence...
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4 downloads reported since publication in 2021.

These counts follow the COUNTER Code of Practice, meaning that Internet robots and repeats within a certain time frame are excluded.
What does this mean?