High-throughput discovery of phage receptors using transposon insertion sequencing of bacteria

Kaitlyn Kortright, Benjamin Chan & Paul Turner
As the most abundant microbes on earth, novel bacteriophage (phage; bacteria-specific viruses) are readily isolated from environmental samples. However, it remains challenging to characterize phage-bacteria interactions, such as the host receptor(s) phage bind to initiate infection. Here, we tested whether transposon insertion sequencing, INSeq, could be used to identify bacterial genes involved in phage binding. As proof of concept, results showed that INSeq screens successfully identified genes encoding known receptors for previously characterized viruses of...
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3 downloads reported since publication in 2020.

These counts follow the COUNTER Code of Practice, meaning that Internet robots and repeats within a certain time frame are excluded.
What does this mean?