Data from: The role of taxonomic expertise in interpretation of metabarcoding studies

Paula Pappalardo, Allen G. Collins, Katrina M. Pagenkopp Lohan, Kate M. Hanson, Sarit B. Truskey, William Jaeckle, Cheryl Lewis Ames, Jessica A. Goodheart, Stephanie L. Bush, Leann M. Biancani, Ellen E. Strong, Michael Vecchione, M. G. Harasewych, Karen Reed, Chan Lin, Elise Hartil, Jessica Whelpley, Jamie Blumberg, Kenan Matterson, Niamh E. Redmond, Allison Becker, Michael J. Boyle & Karen J. Osborn
The performance of DNA metabarcoding approaches for characterizing biodiversity can be influenced by multiple factors. Here we used morphological assessment of taxa in zooplankton samples to develop a large barcode database and to assess the congruence of taxonomic identification with metabarcoding under different conditions. We analyzed taxonomic assignment of metabarcoded samples using two genetic markers (COI, 18S V1-2), two types of clustering into molecular operational taxonomic units (OTUs, ZOTUs), and three methods for taxonomic assignment...
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