This dataset describes the diet of a highly diverse tropical anuran assemblage. We report the diet composition of 35 species collected at Yasuní National Park, based on the examination of the gastrointestinal contents of 396 frog specimens available in the Museo de Zoología (QCAZ) of Pontificia Universidad Católica del Ecuador. Anuran species belong to six families: Aromobatidae, Bufonidae, Dendrobatidae, Hylidae, Leptodactylidae, and Strabomantidae. We were able to identify 4,085 prey items that were classified into...