Environmental DNA (eDNA) sampling can provide accurate, cost-effective, landscape-level data on species distributions. Previous studies have compared the sensitivity of eDNA sampling to traditional sampling methods for single species, but similar comparative studies on multi-species eDNA metabarcoding are rare. Using hierarchical species occupancy-detection models, we examined whether key choices associated with eDNA metabarcoding (primer selection, low-abundance read filtering, and the number of positive water samples used to classify a species as present at a site)...