Validation of an eDNA-based method for the detection of wildlife pathogens in water

Natalie Sieber, Hanna Hartikainen & Christoph Vorburger
Monitoring the occurrence and density of parasites and pathogens can identify high infection-risk areas and facilitates disease control and eradication measures. Environmental DNA (eDNA) techniques are increasingly used for pathogen detection due to their relative ease of application. Since many factors affect the reliability and efficacy of eDNA-based detection, rigorous validation and assessment of method limitations is a crucial first step. We evaluated an eDNA detection method using in-situ filtration of large volume water samples,...
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