Chemical-genetic interrogation of RNA polymerase mutants reveals structure-function relationships and physiological tradeoffs

Anthony Shiver, Hendrik Osadnik, Jason Peters, Rachel Mooney, Peter Wu, Kemardo Henry, Hannes Braberg, Carol Gross, Kerwyn Huang, Robert Landick, Nevan Krogan & James Hu
The multi-subunit bacterial RNA polymerase (RNAP) and its associated regulators carry out transcription and integrate myriad regulatory signals. Numerous studies have interrogated the inner workings of RNAP, and mutations in genes encoding RNAP drive adaptation of Escherichia coli to many health- and industry-relevant environments, yet a paucity of systematic analyses has hampered our understanding of the fitness benefits and trade-offs from altering RNAP function. Here, we conduct a chemical-genetic analysis of a library of RNAP...
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