Validating eDNA Measurements of the Richness and Abundance of Anurans at a Large Scale

Wenhao Li, Xianglei Hou, Chunxia Xu, Mingshuo Qin, Supen Wang, Li Wei, Yanping Wang, Xuan Liu & Yiming Li
In some situations, environmental DNA (eDNA) metabarcoding is a more accurate tool for measuring species richness of a taxon than conventional methods. Whether this tool can reliably estimate the abundance of a taxon remains unresolved. We examined the reliability of metabarcoding for measuring anuran diversity compared to a commonly used traditional line transect method (TLTM) through the replicate sampling of three visits across 71 waterbodies (ponds or reservoirs) in Liuheng, China. We also investigated the...
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