Redox controls RecA protein activity via reversible oxidation of its methionine residues

Benjamin Ezraty, Camille Henry, Frederic Barras, Laurent Loiseau, Alexandra Vergnes, Didier Vertommen, Angela Mérida-Floriano, Sindhu Chitteni-Pattu, Elizabeth Anne Wood, Josep Casadesús & Michael M. Cox
Reactive oxygen species (ROS) cause damage to DNA and proteins. The RecA protein plays a central role in the bacterial response to DNA damage. Here we report that RecA itself is also targeted by ROS. In vivo consequences of RecA oxidation include defects in SOS induction, DNA repair efficiency, and P1 transduction. In vitro, oxidized RecA fails to display ATPase activity, DNA strand exchange capacity and formation of nucleofilaments. Consistently, mass spectrometry analysis of oxidized...
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